Alveolar macrophage cell line is not activated by exposure to polymeric microspheres
Identifieur interne : 002841 ( Main/Exploration ); précédent : 002840; suivant : 002842Alveolar macrophage cell line is not activated by exposure to polymeric microspheres
Auteurs : Ka-Yun Ng [États-Unis] ; Kathleen A. Stringer [États-Unis] ; Zoe Cohen [États-Unis] ; Robert Serravo [États-Unis] ; Bin Tian [États-Unis] ; Jeffrey D. Meyer [États-Unis] ; Richard Falk [États-Unis] ; Theodore Randolph [États-Unis] ; Mark C. Manning [États-Unis] ; David C. Thompson [États-Unis]Source :
- International Journal of Pharmaceutics [ 0378-5173 ] ; 1998.
Descripteurs français
- Pascal (Inist)
- Activation, Alvéole pulmonaire, Animal, Appareil respiratoire, Ciblage, Endocytose, Facteur nécrose tumorale α, Fixation biologique, Forme pharmaceutique, Lactique acide polymère, Lignée cellulaire établie, Macrophage, Microsphère, Polymère vecteur, Précipitation, Rat, Technologie pharmaceutique, Vecteur médicament.
English descriptors
- KwdEn :
- Activation, Alveolar macrophage, Animal, Biological fixation, Control release polymer, Dosage form, Drug carrier, Endocytosis, Established cell line, Lactic acid polymer, Macrophage, Microsphere, NR8383, Oxidative burst and TNF-α, Pharmaceutical technology, Poly(l-lactide) microsphere, Precipitation, Precipitation with compressed anti-solvent, Pulmonary alveolus, Rat, Respiratory system, Targeting, Tumor necrosis factor α.
- Teeft :
- Alveolar macrophage, Alveolar macrophage cell line, Alveolar macrophages, Ammonium chloride, Antitubercular drugs, Cell association, Cell incubator, Cell line, Cells rinsed, Cellular uptake, Chloride solution, Chloroquine, Cluster plate, Colorado health sciences center, Culture medium, Different concentrations, Disease control, Drug therapy, Elsevier science, Endocytosis, Free rhodamine, Important role, International journal, Joint statement, Lysosomotropic, Lysosomotropic agents, Macrophage, Microsphere, Microsphere concentration, Microspheres, Mycobacterium tuberculosis, Opsonized zymosan, Oxidant, Oxidant production, Oxidative, Oxidative burst, Oxidative metabolism, Particle cell interaction, Pharmaceutics, Pulmonary delivery, Rhodamine, Rhodamine microspheres, Scanning electron micrograph, Side effects, Uorescence microscope, Uorescence microscopy, Uptake studies, Zymosan.
Abstract
Abstract: An in vitro cell culture model based on a rat alveolar macrophage (AM) cell line, NR8383, was used to determine if poly(l-lactide) (PLA) microspheres prepared by the precipitation with a compressed antisolvent (PCA) method can be taken up by AMs and activate AMs. To examine cellular uptake of microspheres, microspheres were labeled with rhodamine 6G. Using fluorescence microscopy, the uptake of microspheres by NR8383 cells was followed as a function of time, microsphere concentration, and susceptibility to lysosomotropic agents. To determine if microspheres can activate NR8383 cells, the oxidative burst and production of TNF-α by NR8383 cells following microsphere treatment was measured. Uptake of microspheres by NR8383 cells was dependent on microsphere concentration and appeared to occur via endocytosis, as uptake was significantly inhibited by the putative lysosomotropic agents, ammonium chloride and chloroquine. Furthermore, the microspheres do not appear to activate NR8383 cells, since microsphere exposure results in negligible oxidative burst and TNF-α production in NR8383. Microspheres prepared by the PCA method hold great potential in targeting drugs to AMs and, therefore, may be of utility for the treatment of diseases in which AMs play an important role, such as tuberculosis (TB).
Url:
DOI: 10.1016/S0378-5173(98)00138-0
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000F23
- to stream Istex, to step Curation: 000F23
- to stream Istex, to step Checkpoint: 001645
- to stream Main, to step Merge: 002883
- to stream PascalFrancis, to step Corpus: 000073
- to stream PascalFrancis, to step Curation: 000097
- to stream PascalFrancis, to step Checkpoint: 000081
- to stream Main, to step Merge: 002895
- to stream Main, to step Curation: 002841
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Alveolar macrophage cell line is not activated by exposure to polymeric microspheres</title><author><name sortKey="Ng, Ka Yun" sort="Ng, Ka Yun" uniqKey="Ng K" first="Ka-Yun" last="Ng">Ka-Yun Ng</name></author><author><name sortKey="Stringer, Kathleen A" sort="Stringer, Kathleen A" uniqKey="Stringer K" first="Kathleen A." last="Stringer">Kathleen A. Stringer</name></author><author><name sortKey="Cohen, Zoe" sort="Cohen, Zoe" uniqKey="Cohen Z" first="Zoe" last="Cohen">Zoe Cohen</name></author><author><name sortKey="Serravo, Robert" sort="Serravo, Robert" uniqKey="Serravo R" first="Robert" last="Serravo">Robert Serravo</name></author><author><name sortKey="Tian, Bin" sort="Tian, Bin" uniqKey="Tian B" first="Bin" last="Tian">Bin Tian</name></author><author><name sortKey="Meyer, Jeffrey D" sort="Meyer, Jeffrey D" uniqKey="Meyer J" first="Jeffrey D." last="Meyer">Jeffrey D. Meyer</name></author><author><name sortKey="Falk, Richard" sort="Falk, Richard" uniqKey="Falk R" first="Richard" last="Falk">Richard Falk</name></author><author><name sortKey="Randolph, Theodore" sort="Randolph, Theodore" uniqKey="Randolph T" first="Theodore" last="Randolph">Theodore Randolph</name></author><author><name sortKey="Manning, Mark C" sort="Manning, Mark C" uniqKey="Manning M" first="Mark C." last="Manning">Mark C. Manning</name></author><author><name sortKey="Thompson, David C" sort="Thompson, David C" uniqKey="Thompson D" first="David C." last="Thompson">David C. Thompson</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:35C565B5CF0706547DC94D5F27F45E742E40D1D6</idno><date when="1998" year="1998">1998</date><idno type="doi">10.1016/S0378-5173(98)00138-0</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-TMN14T7Q-7/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000F23</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000F23</idno><idno type="wicri:Area/Istex/Curation">000F23</idno><idno type="wicri:Area/Istex/Checkpoint">001645</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001645</idno><idno type="wicri:doubleKey">0378-5173:1998:Ng K:alveolar:macrophage:cell</idno><idno type="wicri:Area/Main/Merge">002883</idno><idno type="wicri:source">INIST</idno><idno type="RBID">Pascal:98-0509768</idno><idno type="wicri:Area/PascalFrancis/Corpus">000073</idno><idno type="wicri:Area/PascalFrancis/Curation">000097</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000081</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000081</idno><idno type="wicri:doubleKey">0378-5173:1998:Ng K:alveolar:macrophage:cell</idno><idno type="wicri:Area/Main/Merge">002895</idno><idno type="wicri:Area/Main/Curation">002841</idno><idno type="wicri:Area/Main/Exploration">002841</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Alveolar macrophage cell line is not activated by exposure to polymeric microspheres</title><author><name sortKey="Ng, Ka Yun" sort="Ng, Ka Yun" uniqKey="Ng K" first="Ka-Yun" last="Ng">Ka-Yun Ng</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pharmaceutical Sciences, School of Pharmacy, Campus Box C-238, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">États-Unis</country></affiliation></author><author><name sortKey="Stringer, Kathleen A" sort="Stringer, Kathleen A" uniqKey="Stringer K" first="Kathleen A." last="Stringer">Kathleen A. Stringer</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pharmacy Practice, School of Pharmacy, University of Colorado Health Sciences Center, Denver, CO 80262</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation></author><author><name sortKey="Cohen, Zoe" sort="Cohen, Zoe" uniqKey="Cohen Z" first="Zoe" last="Cohen">Zoe Cohen</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pharmacy Practice, School of Pharmacy, University of Colorado Health Sciences Center, Denver, CO 80262</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation></author><author><name sortKey="Serravo, Robert" sort="Serravo, Robert" uniqKey="Serravo R" first="Robert" last="Serravo">Robert Serravo</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pharmaceutical Sciences, School of Pharmacy, Campus Box C-238, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation></author><author><name sortKey="Tian, Bin" sort="Tian, Bin" uniqKey="Tian B" first="Bin" last="Tian">Bin Tian</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pharmaceutical Sciences, School of Pharmacy, Campus Box C-238, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation></author><author><name sortKey="Meyer, Jeffrey D" sort="Meyer, Jeffrey D" uniqKey="Meyer J" first="Jeffrey D." last="Meyer">Jeffrey D. Meyer</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pharmaceutical Sciences, School of Pharmacy, Campus Box C-238, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation></author><author><name sortKey="Falk, Richard" sort="Falk, Richard" uniqKey="Falk R" first="Richard" last="Falk">Richard Falk</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical Engineering, University of Colorado at Boulder, Boulder, CO 80309</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation></author><author><name sortKey="Randolph, Theodore" sort="Randolph, Theodore" uniqKey="Randolph T" first="Theodore" last="Randolph">Theodore Randolph</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical Engineering, University of Colorado at Boulder, Boulder, CO 80309</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation></author><author><name sortKey="Manning, Mark C" sort="Manning, Mark C" uniqKey="Manning M" first="Mark C." last="Manning">Mark C. Manning</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pharmaceutical Sciences, School of Pharmacy, Campus Box C-238, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation></author><author><name sortKey="Thompson, David C" sort="Thompson, David C" uniqKey="Thompson D" first="David C." last="Thompson">David C. Thompson</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pharmaceutical Sciences, School of Pharmacy, Campus Box C-238, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">International Journal of Pharmaceutics</title><title level="j" type="abbrev">IJP</title><idno type="ISSN">0378-5173</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">170</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="41">41</biblScope><biblScope unit="page" to="49">49</biblScope></imprint><idno type="ISSN">0378-5173</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0378-5173</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Activation</term><term>Alveolar macrophage</term><term>Animal</term><term>Biological fixation</term><term>Control release polymer</term><term>Dosage form</term><term>Drug carrier</term><term>Endocytosis</term><term>Established cell line</term><term>Lactic acid polymer</term><term>Macrophage</term><term>Microsphere</term><term>NR8383</term><term>Oxidative burst and TNF-α</term><term>Pharmaceutical technology</term><term>Poly(l-lactide) microsphere</term><term>Precipitation</term><term>Precipitation with compressed anti-solvent</term><term>Pulmonary alveolus</term><term>Rat</term><term>Respiratory system</term><term>Targeting</term><term>Tumor necrosis factor α</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Activation</term><term>Alvéole pulmonaire</term><term>Animal</term><term>Appareil respiratoire</term><term>Ciblage</term><term>Endocytose</term><term>Facteur nécrose tumorale α</term><term>Fixation biologique</term><term>Forme pharmaceutique</term><term>Lactique acide polymère</term><term>Lignée cellulaire établie</term><term>Macrophage</term><term>Microsphère</term><term>Polymère vecteur</term><term>Précipitation</term><term>Rat</term><term>Technologie pharmaceutique</term><term>Vecteur médicament</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Alveolar macrophage</term><term>Alveolar macrophage cell line</term><term>Alveolar macrophages</term><term>Ammonium chloride</term><term>Antitubercular drugs</term><term>Cell association</term><term>Cell incubator</term><term>Cell line</term><term>Cells rinsed</term><term>Cellular uptake</term><term>Chloride solution</term><term>Chloroquine</term><term>Cluster plate</term><term>Colorado health sciences center</term><term>Culture medium</term><term>Different concentrations</term><term>Disease control</term><term>Drug therapy</term><term>Elsevier science</term><term>Endocytosis</term><term>Free rhodamine</term><term>Important role</term><term>International journal</term><term>Joint statement</term><term>Lysosomotropic</term><term>Lysosomotropic agents</term><term>Macrophage</term><term>Microsphere</term><term>Microsphere concentration</term><term>Microspheres</term><term>Mycobacterium tuberculosis</term><term>Opsonized zymosan</term><term>Oxidant</term><term>Oxidant production</term><term>Oxidative</term><term>Oxidative burst</term><term>Oxidative metabolism</term><term>Particle cell interaction</term><term>Pharmaceutics</term><term>Pulmonary delivery</term><term>Rhodamine</term><term>Rhodamine microspheres</term><term>Scanning electron micrograph</term><term>Side effects</term><term>Uorescence microscope</term><term>Uorescence microscopy</term><term>Uptake studies</term><term>Zymosan</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: An in vitro cell culture model based on a rat alveolar macrophage (AM) cell line, NR8383, was used to determine if poly(l-lactide) (PLA) microspheres prepared by the precipitation with a compressed antisolvent (PCA) method can be taken up by AMs and activate AMs. To examine cellular uptake of microspheres, microspheres were labeled with rhodamine 6G. Using fluorescence microscopy, the uptake of microspheres by NR8383 cells was followed as a function of time, microsphere concentration, and susceptibility to lysosomotropic agents. To determine if microspheres can activate NR8383 cells, the oxidative burst and production of TNF-α by NR8383 cells following microsphere treatment was measured. Uptake of microspheres by NR8383 cells was dependent on microsphere concentration and appeared to occur via endocytosis, as uptake was significantly inhibited by the putative lysosomotropic agents, ammonium chloride and chloroquine. Furthermore, the microspheres do not appear to activate NR8383 cells, since microsphere exposure results in negligible oxidative burst and TNF-α production in NR8383. Microspheres prepared by the PCA method hold great potential in targeting drugs to AMs and, therefore, may be of utility for the treatment of diseases in which AMs play an important role, such as tuberculosis (TB).</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Colorado</li></region></list><tree><country name="États-Unis"><region name="Colorado"><name sortKey="Ng, Ka Yun" sort="Ng, Ka Yun" uniqKey="Ng K" first="Ka-Yun" last="Ng">Ka-Yun Ng</name></region><name sortKey="Cohen, Zoe" sort="Cohen, Zoe" uniqKey="Cohen Z" first="Zoe" last="Cohen">Zoe Cohen</name><name sortKey="Falk, Richard" sort="Falk, Richard" uniqKey="Falk R" first="Richard" last="Falk">Richard Falk</name><name sortKey="Manning, Mark C" sort="Manning, Mark C" uniqKey="Manning M" first="Mark C." last="Manning">Mark C. Manning</name><name sortKey="Meyer, Jeffrey D" sort="Meyer, Jeffrey D" uniqKey="Meyer J" first="Jeffrey D." last="Meyer">Jeffrey D. Meyer</name><name sortKey="Ng, Ka Yun" sort="Ng, Ka Yun" uniqKey="Ng K" first="Ka-Yun" last="Ng">Ka-Yun Ng</name><name sortKey="Randolph, Theodore" sort="Randolph, Theodore" uniqKey="Randolph T" first="Theodore" last="Randolph">Theodore Randolph</name><name sortKey="Serravo, Robert" sort="Serravo, Robert" uniqKey="Serravo R" first="Robert" last="Serravo">Robert Serravo</name><name sortKey="Stringer, Kathleen A" sort="Stringer, Kathleen A" uniqKey="Stringer K" first="Kathleen A." last="Stringer">Kathleen A. Stringer</name><name sortKey="Thompson, David C" sort="Thompson, David C" uniqKey="Thompson D" first="David C." last="Thompson">David C. Thompson</name><name sortKey="Tian, Bin" sort="Tian, Bin" uniqKey="Tian B" first="Bin" last="Tian">Bin Tian</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002841 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002841 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:35C565B5CF0706547DC94D5F27F45E742E40D1D6
|texte= Alveolar macrophage cell line is not activated by exposure to polymeric microspheres
}}
| This area was generated with Dilib version V0.6.33. |  |